Description

The practical work consists of acquiring an essential stage of morphogenesis in zebrafish. After acquisition, the images are aligned, segmented, analysed, and compared to a theoretical model.

Compétences visées

- Prepare a sample of living material.
- Acquire data under a microscope.
- Identify relevant output readings.
- Quantify.
- Compare with a model and propose biological functions.

Modalités d'organisation et de suivi

Remise d'un rapport 15 jours après les TPs.

Syllabus

1) Optical microscopy : epifluorescence/spinning disk confocal
2) 2 photon microscopy
3) FRAP/FRET
4) Super-resolution
5) Electron microscopy : basics